Leeper Group



C. E. Goodwin and F. J. Leeper
"Stereochemistry and Mechanism of the Conversion of 5-Aminolaevulinic Acid into Porphobilinogen Catalysed by Porphobilinogen Synthase."
Org. Biomolec. Chem.,, 2003, 1, 1443-1446. Full Text

(3R)- and (3S)-Deuteriated forms of 5-aminolaevulinic acid have been synthesised and the (3R)-form shows a significantly larger isotope effect when incubated with porphobilinogen synthase from bovine liver and from Bacillus subtilis; based on this and on available crystal structures, a modified mechanism for the enzymic reaction is proposed.

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Department of Chemistry
University of Cambridge