K. Ichinose, F. J. Leeper and A. R. Battersby
"Preparation of [4R-3H]NADH, [4R-3H]NADPH and the Corresponding 4S-Isomers All with Substantial Specific Activities"
J. Chem. Soc., Perkin Trans. 1, 1993, 1213-1216. Full Text
Methods are developed for the enzymic preparation of [4R-3H]NADH, [4R-3H]NADPH and the corresponding 4S-isomers, all stereospecifically labelled and of specific activities amply high enough for sensitive labelling experiments. Sodium borotritiide provides the tritium for all four cofactors.
Department of Chemistry
University of Cambridge