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Abstract 141 Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bio-orthogonal isonitrile–tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.
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Shaun Stairs, Andre A. Neves, Henning Stöckmann, Yelena A. Wainman,
Heather Ireland-Zecchini, Kevin M. Brindle, and Finian J. Leeper,